肝肿瘤·血管内皮生长因子·明胶酶β·微血管密度·肿瘤转移

血管内皮生长因子和基质金属蛋白酶9在原发性肝细胞癌中的表达及其临床意义

http://www.100md.com 《中国现代普通外科进展》 2006年第5期

肝肿瘤·血管内皮生长因子·明胶酶β·微血管密度·肿瘤转移,,肝肿瘤·血管内皮生长因子·明胶酶β·微血管密度·肿瘤转移,1

     【摘要】目的：探讨血管内皮生长因子(VEGF)和基质金属蛋白酶9(MMP9)的表达与原发性肝细胞癌(PHCC)侵袭和转移的关系。方法：采用免疫组织化学LSAB法和原位杂交法对40例PHCC和癌旁组织手术切除后的石蜡标本组织中的VEGF和MMP9的表达及微血管密度(MVD)进行了检测。结果：①VEGF、MMP9表达与MVD、PHCC组织中明显高于癌旁组织；②在PHCC中，有转移者及包膜不完整者VEGF、MMP9表达与MVD明显高于无转移者及包膜完整者；③VEGF、MMP9表达与MVD在大肿瘤(直径>5cm)与小肿瘤(直径≤5cm)两者差异无统计学意义；④VEGF的表达与MVD呈正相关。结论：原发性肝细胞癌组织中的VEGF、MMP9表达与肿瘤的侵袭和转移行为密切相关，可作为判定HCC发生、转移及预后的指标。

    【关键词】肝肿瘤·血管内皮生长因子·明胶酶β·微血管密度·肿瘤转移

    The expression and clinical significance of VEGF and MMP9 in primary hepatocellular carcinoma

    ZHOU Tianbao,YANG Guangshun

    Department of Biliary Tract Surgery,Easten Hepatobiliary Surgery Hospital Attached to the second Military Medical University(Shanghai 200433,China)

    【ABSTRACT】Objective:To study the relationship between the expression of VEGF and MMP9 with invasion,metastasis of primary hepatocellular carcinoma(PHCC).Methods: Paraffinembedded specimens from 40 patients with PHCC undergoing radical resection were studied.Agiogenesis was assessed by the expression of VEGF,MMP9 and MVD using LSAB immunohistochemical staining and in situ hybridization.Results:①The level of VEGF,MMP9 expression and MVD in PHCC was higher than that in the paratumorous tissue(P<0.01).②The level of VEGF,MMP9 expression and MVD in tumor with tumor metastasis or in nonencapsulated tumors were higher than that in tumors without metastasis or in wellencapsulated tumors(P<0.05).③There was no significant difference in the level of VEGF,MMP9 expression and MVD between large PHCC(diameter >5cm) and small ones (diameter≤5cm)(P>0.05).④The expression of VEGF was positively correlated with MVD(P<0.01).Conclusion:The expression of VEGF,MMP9 and MVD are highly correlated with invasion and metastasis in PHCC,which may serve as a parameter for determining tumor metastasis and prognosis.It may be a new idea to supervise and treat HCC. ......

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